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HPLC of sialooligosaccharides and gangliosides
Summary
Type:
HPLC
Application #:
102740
Detection:
UV, 195nm
Conditions:
Fig. 2: ; eluent: 15mM phosphate buffer, pH 3.2, and 10mM phosphate, pH 6.3, respectively
flow rate 1.0ml
temperature 25°C
Fig. 5: ; eluent: 10mM phosphate, pH 4.0
flow rate 1.0ml
temperature 25°C
Authors:
el Rassi Z
Source:
J Chromatogr 488 (1989) 229 - 236
Details
Legend: Fig. 2. Separation of ganglioside oligosaccharides. GA1 = asialo-, GM1 = monosialo-, GD1a and GD1b = disialo-, GT1b = trisialooligosaccharide. Fig. 5. Separation of N-acetylneuraminic (NANA) and N
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